Description:
NRF2/ARE Responsive Luciferase Reporter NIH3T3 Stable Cell Line is derived from Mouse fibroblast, and stably express firefly luciferase reporter gene under the control of the NRF2/ARE response element. This cell line is an ideal cellular model for monitoring the activation of Antioxidant Response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.Principle:
NRF2 plays a crucial role in cellular anti-oxidant defense, making it a therapeutic target for neurodegenerative diseases and cancer. Under normal conditions, NRF2 localizes in the cytosol and is rapidly degraded by the proteasome. Under oxidative stress, NRF2 is stabilized and translocates to the nucleus where it binds to a DNA promoter and initiates gene expression. In the nucleus, NRF2 forms a heterodimer with a small Maf protein and binds to the Antioxidant Response Element in the upstream promoter region of many antioxidative genes, and initiates their transcription.
This NRF2 luciferase reporter stable cell line has been stably transfected with pTA-ARE-luciferase reporter vector, which contains 4 repeats of antioxidant response binding sites, a minimal promoter upstream of the firefly luciferase coding region, along with a hygromycin expression vector. Following selection, the hygromycin resistant clones were subsequently screened for TBHQ-induced luciferase activity. The clone with the highest fold induction was selected and expanded to produce this stable cell line.
|
Principle behind TF luciferase reporter. TF luciferase reporter stable cell line utilizes artificial promoter constructs to drive luciferase expression. The promoter region can consists of multiple repeats of a cis-element TF binding site, a DNA fragment from the promoter region of a known TF downstream gene, or a DNA fragment containing putative/known TF binding sites. There are several ways that a TF can be activated, such as through extracellular stimuli or through intracellular signaling pathways. Once activated, the TF translocates to the nucleus and often interacts with relevant co-factors to drive gene expression. Once luciferase is expressed, it can generate light in an enzymatic assay and the amount of light measured is positively correlated with the level of TF activation. |
Data:
Analysis of the NRF2 Pathway Reporter NIH3T3 Stable Cell Line in response to stimuli. The cells were seeded on a 96-well plate for 8 hours or overnight with DMEM including 10% FBS. The cells then were treated with or without 10μM TBHQ or 10μM 4HNE respectively in DMEM and 0.1% FBS for 16 hours.
Signosis
Signosis提供一种分子工具的公司，研发独特的产品用于生命科学研究，临床和药物发现研究领域中生物标志物的发现和分析。
Signosis is a leader of bioassay provider over a decade, dedicated to the development and commercialization of plate-based analysis products in the life sciences community. We focus on many regulatory key molecules: transcription factors, cytokines, miRNAs, and disease markers. Our mission is to provide excellent products and service worldwide, which include scientists in pharmaceutical companies and biotech industry, academic and research institutes in many countries. We object to develop innovative assay products with high sensitivity, high throughput and cost-effectiveness.
产品列表：
No. | 品牌 | 货号 | 名称 | 规格 |
1 | Signosis | p300 Microplate Snapshot ChIP Assay | ||
2 | Signosis | Human NFκB-Regulated cDNA Plate Array | ||
3 | Signosis | Human miRNA Array I | ||
4 | Signosis | Snapshot ChIP Assay | ||
5 | Signosis | OCT4 Snapshot ChIP Assay | ||
6 | Signosis | Microplate Snapshot ChIP Assay | ||
7 | Signosis | NRF2 Microplate Snapshot ChIP Assay | ||
8 | Signosis | SMAD Microplate Snapshot ChIP Assay | ||
9 | Signosis | NFkB Microplate Snapshot ChIP Assay | ||
10 | Signosis | H3 Microplate Snapshot ChIP Assay | ||
11 | Signosis | Human NFκB-Regulated cDNA Profiling in Cell Lysates | ||
12 | Signosis | Human Apoptosis cDNA Plate Array I | ||
13 | Signosis | AP2 EMSA Kit | ||
14 | Signosis | NRF2 Snapshot ChIP Assay | ||
15 | Signosis | FA-0004 | AP1 Filter Plate Assay |
本网站将在规定时间内给予删除等相关处理。
