Description:

Angiogenesis provides the crucial link between the avascular and vascular states and is a key event for sustained tumor growth and cancer progression. Angiogenesis as a biological switch process is governed by numerous pro- and anti-angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor (FGFb), epidermal growth factor (EGF), and transforming growth factor-beta (TGF-b). The mechanism of action of each of these factors is different, as are their origin and the stimuli for their production. The angiogenic switch refers to the balance between pro- and anti- angiogenic factors. Therefore, profiling of these factors is critical to understanding angiogenesis. Signosis’ Angiogenesis ELISA Strip I Profiling Assay allows simultaneously profiling 8 angiogenesis cytokines. Each well of the strip is coated with a primary antibody against a specific angiogenesis protein and total 8 wells of a strip target 8 different proteins. The difference of these proteins between two samples can be determined through data comparison.

Principle:

Each well of the strip is coated with a specific capture antibody to detect its corresponding cytokine in the sample. Therefore, 8 different proteins can be measures simultaneously. The test sample reacts simultaneously with two antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. The HRP substrate, TMB, is then added and causes a blue color change. The reaction is then terminated with Stop Solution, resulting in a yellow color. The concentrations of oxidative stress cytokines are directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. The expression levels of these cytokines can be quantitatively compared between samples.

 

 

 

Data:

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Human Angiogenesis ELISA strip I for profiling 8 cytokines. HeLa cells were treated with (red bars) or without (light blue) 10ng/ml TNF. Conditioned media were collected (top) and cell lysates were prepared (bottom) for ELISA strip profiling.

 

 

Signosis

Signosis提供一种分子工具的公司，研发独特的产品用于生命科学研究，临床和药物发现研究领域中生物标志物的发现和分析。

Signosis is a leader of bioassay provider over a decade, dedicated to the development and commercialization of plate-based analysis products in the life sciences community. We focus on many regulatory key molecules: transcription factors, cytokines, miRNAs, and disease markers. Our mission is to provide excellent products and service worldwide, which include scientists in pharmaceutical companies and biotech industry, academic and research institutes in many countries. We object to develop innovative assay products with high sensitivity,  high throughput and cost-effectiveness.

产品列表：

No.	品牌	货号	名称	规格
1	Signosis	CP-0109S	p300 Microplate Snapshot ChIP Assay
2	Signosis	AP-0101	Human NFκB-Regulated cDNA Plate Array
3	Signosis	AP-0001	Human miRNA Array I
4	Signosis	CA-0001	Snapshot ChIP Assay
5	Signosis	CA-0101	OCT4 Snapshot ChIP Assay
6	Signosis	CP-0001S	Microplate Snapshot ChIP Assay
7	Signosis	CP-0102S	NRF2 Microplate Snapshot ChIP Assay
8	Signosis	CP-0104S	SMAD Microplate Snapshot ChIP Assay
9	Signosis	CP-0106S	NFkB Microplate Snapshot ChIP Assay
10	Signosis	CP-0108S	H3 Microplate Snapshot ChIP Assay
11	Signosis	AP-3101	Human NFκB-Regulated cDNA Profiling in Cell Lysates
12	Signosis	AP-0121	Human Apoptosis cDNA Plate Array I
13	Signosis	GS-0002	AP2 EMSA Kit
14	Signosis	CA-0102	NRF2 Snapshot ChIP Assay
15	Signosis	FA-0004	AP1 Filter Plate Assay