Description:

Principle:

Mouse CytokineELISAisbasedontheprincipleofasolidphaseenzyme-linkedimmunosorbentassay.Theassayutilizesrabbitanti-mouseantibodiesforimmobilizationonthemicrotiterwellsandrabbitanti-mouseantibodiesalongwithstreptavidinconjugatedtohorserADIshperoxidase(HRP)fordetection.Thetestsampleisallowedtoreactsimultaneouslywiththetwoantibodies,resultinginthemoleculesbeingsandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Afterincubation,thewellsarewashedtoremoveunbound-labeledantibodies.AHRPsubstrate,TMB,isaddedtoresultinthedevelopmentofabluecolor.ThecolordevelopmentisthenstoppedwiththeadditionofStopSolutionchangingthecolortoyellow.The  concentrationofVEGFisdirectlyproportionaltothecolorintensityofthetestsample.Absorbanceismeasuredspectrophotometricallyat450nm. 
 

Data:

Signosis

Signosis提供一种分子工具的公司，研发独特的产品用于生命科学研究，临床和药物发现研究领域中生物标志物的发现和分析。

Signosis is a leader of bioassay provider over a decade, dedicated to the development and commercialization of plate-based analysis products in the life sciences community. We focus on many regulatory key molecules: transcription factors, cytokines, miRNAs, and disease markers. Our mission is to provide excellent products and service worldwide, which include scientists in pharmaceutical companies and biotech industry, academic and research institutes in many countries. We object to develop innovative assay products with high sensitivity,  high throughput and cost-effectiveness.

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