Description:

Anti-nuclear antibodies (ANA) are a group of antibodies directed against various nuclear and some cytoplasmic antigens. Serological tests for ANA play an important role towards the diagnosis of various autoimmune connective tissue disorders. Although these antibodies were first associated with systemic lupus erythematosus (SLE), the list of implicated diseases has expanded and many rheumatic diseases are characterized by the presence of one or more of these ANAs. For instance, anti-SSA/Ro and anti-SSB/La antibodies are associated with SLE and Sjogren's Syndrome (SS), anti-dsDNA and anti-Sm antibodies with SLE, anti-RNP antibodies with mixed connective tissue disease (MCTD) and SLE, anti-Scl-70 antibodies with scleroderma (progressive systemic sclerosis (PSSJ), anti-Jo1 with polymyositis and dermatomyositis and anti-centromere antibodies with CREST syndrome. As ANA ELISA test collectively detects, in one well, total ANAs against double stranded DNA (dsDNA), Sm, U1-RNP (68K), SS-A/Ro, SS-B/La, Scl-70, Jo-1, and centromeric antigens, along with sera positive for IFA HEp-2 ANAs, it is not specific indicators of a connective tissue disease. To monitor more specific antibodies, eight different antigens (dsDNA, SmD1, U1-RNA (68K), SS-A/Ro, SS-B/La, Scl-70, Jo-1, and CENP-B) are coated to different wells in a column or strip for the ELISA screen test of eight different autoimmune antibodies once.

Applicable Grid:

 

List of Applicable ANAs

dsDNA

SmD1

U1-RNA

SS-A/Ro

SS-B/La

Scl-70

Jo-1

CENP-B

Principle:

Autoimmune ELISA kits measure autoimmune antibodies in the serum. It is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes a specific antigen for immobilization on the microtiter wells and anti-human IgG antibody conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two components, resulting in autoimmune antibodies being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentration of autoimmune antibodies is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

 

 

 

Data:

 

 

 

Signosis

Signosis提供一种分子工具的公司，研发独特的产品用于生命科学研究，临床和药物发现研究领域中生物标志物的发现和分析。

Signosis is a leader of bioassay provider over a decade, dedicated to the development and commercialization of plate-based analysis products in the life sciences community. We focus on many regulatory key molecules: transcription factors, cytokines, miRNAs, and disease markers. Our mission is to provide excellent products and service worldwide, which include scientists in pharmaceutical companies and biotech industry, academic and research institutes in many countries. We object to develop innovative assay products with high sensitivity,  high throughput and cost-effectiveness.

产品列表：

No.	品牌	货号	名称	规格
1	Signosis	CP-0109S	p300 Microplate Snapshot ChIP Assay
2	Signosis	AP-0101	Human NFκB-Regulated cDNA Plate Array
3	Signosis	AP-0001	Human miRNA Array I
4	Signosis	CA-0001	Snapshot ChIP Assay
5	Signosis	CA-0101	OCT4 Snapshot ChIP Assay
6	Signosis	CP-0001S	Microplate Snapshot ChIP Assay
7	Signosis	CP-0102S	NRF2 Microplate Snapshot ChIP Assay
8	Signosis	CP-0104S	SMAD Microplate Snapshot ChIP Assay
9	Signosis	CP-0106S	NFkB Microplate Snapshot ChIP Assay
10	Signosis	CP-0108S	H3 Microplate Snapshot ChIP Assay
11	Signosis	AP-3101	Human NFκB-Regulated cDNA Profiling in Cell Lysates
12	Signosis	AP-0121	Human Apoptosis cDNA Plate Array I
13	Signosis	GS-0002	AP2 EMSA Kit
14	Signosis	CA-0102	NRF2 Snapshot ChIP Assay
15	Signosis	FA-0004	AP1 Filter Plate Assay