Description:

Principle:

Beta-actin ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes a rabbit polyclonal beta-actin capture antibody for immobilization on the microtiter wells, and a mouse monoclonal beta-actin detection antibody along with anti-mouse conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the beta-actin molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of stop solution changing the color to yellow. The concentration of beta-actin is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

Data:

Signosis

Signosis提供一种分子工具的公司，研发独特的产品用于生命科学研究，临床和药物发现研究领域中生物标志物的发现和分析。

Signosis is a leader of bioassay provider over a decade, dedicated to the development and commercialization of plate-based analysis products in the life sciences community. We focus on many regulatory key molecules: transcription factors, cytokines, miRNAs, and disease markers. Our mission is to provide excellent products and service worldwide, which include scientists in pharmaceutical companies and biotech industry, academic and research institutes in many countries. We object to develop innovative assay products with high sensitivity,  high throughput and cost-effectiveness.

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