Description:

Oxidative stress can directly damage cell structures and may lead to cancer, inflammatory diseases, and aging. Interestingly, oxidative stress can also alter the expression levels of a variety of cytokines. Understanding the conditions that alter the expression of these cellular messengers is important for unraveling the mechanisms of these diseases and developing therapeutics.  Signosis’s Oxidative Stress ELISA Strip Profiling Assay can simultaneously analyze 8 cytokines.  Each well of the strip is coated with a specific capture antibody to detect corresponding cytokine in the sample. The expression levels of these cytokines can be quantitatively compared between samples.

Principle:

Each well of the strip is coated with a specific capture antibody to detect its corresponding cytokine in the sample. Therefore, 8 different proteins can be measures simultaneously. The test sample reacts simultaneously with two antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. The HRP substrate, TMB, is then added and causes a blue color change. The reaction is then terminated with Stop Solution, resulting in a yellow color. The concentrations of oxidative stress cytokines are directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. The expression levels of these cytokines can be quantitatively compared between samples.

 

 

 

 

 

 

Data:

 

 

 

Three different patient samples were analyzed using the Human Oxidative Stress ELISA Strip for Profiling 8 Cytokines.

Signosis

Signosis提供一种分子工具的公司，研发独特的产品用于生命科学研究，临床和药物发现研究领域中生物标志物的发现和分析。

Signosis is a leader of bioassay provider over a decade, dedicated to the development and commercialization of plate-based analysis products in the life sciences community. We focus on many regulatory key molecules: transcription factors, cytokines, miRNAs, and disease markers. Our mission is to provide excellent products and service worldwide, which include scientists in pharmaceutical companies and biotech industry, academic and research institutes in many countries. We object to develop innovative assay products with high sensitivity,  high throughput and cost-effectiveness.

产品列表：

No.	品牌	货号	名称	规格
1	Signosis	CP-0109S	p300 Microplate Snapshot ChIP Assay
2	Signosis	AP-0101	Human NFκB-Regulated cDNA Plate Array
3	Signosis	AP-0001	Human miRNA Array I
4	Signosis	CA-0001	Snapshot ChIP Assay
5	Signosis	CA-0101	OCT4 Snapshot ChIP Assay
6	Signosis	CP-0001S	Microplate Snapshot ChIP Assay
7	Signosis	CP-0102S	NRF2 Microplate Snapshot ChIP Assay
8	Signosis	CP-0104S	SMAD Microplate Snapshot ChIP Assay
9	Signosis	CP-0106S	NFkB Microplate Snapshot ChIP Assay
10	Signosis	CP-0108S	H3 Microplate Snapshot ChIP Assay
11	Signosis	AP-3101	Human NFκB-Regulated cDNA Profiling in Cell Lysates
12	Signosis	AP-0121	Human Apoptosis cDNA Plate Array I
13	Signosis	GS-0002	AP2 EMSA Kit
14	Signosis	CA-0102	NRF2 Snapshot ChIP Assay
15	Signosis	FA-0004	AP1 Filter Plate Assay